The genome of Border disease virus genotype 8 from chamois by next generation sequencing

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Cerutti, F., Caruso, C., Modesto, P., Orusa, R., Masoero, L., Acutis, P. L., & Peletto, S. (2019). The genome of Border disease virus genotype 8 from chamois by next generation sequencing. Veterinaria Italiana, 55(1), 103–105. https://doi.org/10.12834/VetIt.1768.9338.1


Border disease virus (BDV) is widespread both in domestic small ruminants and wildlife. Here we report the genome of BDV genotype 8 from chamois, strain Italy‑58987, obtained by next generation sequencing and the comparison with other pestiviruses. The sequence of 12,245 bp long was aligned to 22 pestivirus genomes and it showed a nt/aa similarity of 81.3/89.4% with BDV genotypes, and 65.9/67.8% with the other  pestiviruses. The genome showed a mean nt/aa similarity of 91.2/95.0% with three Swiss genomes closely related to the BDV‑8 5’‑UTR and Npro sequences. The identification of divergent BDV‑8 isolates in North‑Western Italy and in Switzerland suggests that this genotype may have been circulating in a wider area than previously supposed, and may have a high host adaptability.


Caruso C., Peletto S., Cerutti F., Modesto P., Robetto S., Domenis L., Masoero L. & Acutis P.L. 2016. Evidence of circulation of the novel border disease virus genotype 8 in chamois. Arch. Virol. 1–5.

Edgar R.C. 2004. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32, 1792–1797.

Giammarioli M., La Rocca S.A., Steinbach F., Casciari C. & De Mia G.M. 2011. Genetic and antigenic typing of border disease virus (BDV) isolates from Italy reveals the existence of a novel BDV group. Vet. Microbiol. 147, 231–236.

Li D., Luo R., Liu C.M., Leung C.M., Ting H.F., Sadakane K., Yamashita H. & Lam T.W. 2016. MEGAHIT v1.0: A fast and scalable metagenome assembler driven by advanced methodologies and community practices. Methods 102, 3–11.

Peletto S., Caruso C., Cerutti F., Modesto P., Zoppi S., Dondo A., Acutis P.L. & Masoero L. 2016. A new genotype of border disease virus with implications for molecular diagnostics. Arch. Virol. 161, 471–477.

Ronquist F., Teslenko M., van der Mark P., Ayres D.L., Darling A., Höhna S., Larget B., Liu L., Suchard M.A. & Huelsenbeck J.P. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61, 539–542.

Smith D.B., Meyers G., Bukh J., Gould E.A., Monath T., Scott Muerhoff A., Pletnev A., Rico-Hesse R., Stapleton J.T., Simmonds P. & Becher P. 2017. Proposed revision to the taxonomy of the genus Pestivirus, family Flaviviridae. J. Gen. Virol. 1–7.

Stalder H., Marti S., Flückiger F., Renevey N., Hofmann M.A. & Schweizer M. 2017. Complete Genome Sequences of Three Border Disease Virus Strains of the Same Subgenotype, BDSwiss, Isolated from Sheep, Cattle, and Pigs in Switzerland. Genome Announc. 5, e01238-17.