Subcutaneous phaeohyphomycosis due to Aureobasidium pullulans infection in a dog


Aureobasidium pullulans
Black yeasts
Dematiaceous fungus
Differential diagnosis
Opportunistic infection

How to Cite

Waller, S. B., Ferreira, M. R. A., Gomes, A. dos R., Ripoll, M. K., Silva, A. L., Martins, O. de A., Osorio, L. da G., Conceição, F. R., de Faria, R. O., & Meireles, M. C. A. (2021). Subcutaneous phaeohyphomycosis due to Aureobasidium pullulans infection in a dog. Veterinaria Italiana, 57(3). https://doi.org/10.12834/VetIt.1821.9620.3


A case of subcutaneous phaeohyphomycosis in a dog with an ulcerative lesion on the right limb during a post-operative period of castration was described for the first time. The macroscopic and microscopic characteristics of the fungal colonies growth on the Sabouraud‑dextrose agar were detailed. The fungus was identified as Aureobasidium pullulans on the basis of the phenotypic analysis, which was confirmed by sequencing of the internal transcribed spacers (ITS) region of rDNA. The patient might have acquired the infection through traumatic inoculation by environmental contact, along with the immunological condition during the stressful period of postoperative. The spontaneous remission of the lesion was observed in five weeks without antifungal treatment. This work highlights the importance of considering the pathogenic potential of this environmental fungus and the need of including it in the differential diagnosis of cutaneous lesions in dogs.



Angele M.K. & Faist. E. 2002. Clinical review: immunodepression in the surgical patient and increased susceptibility to infection. Crit Care, 6, 298–305.

Bernhardt A., von Bornhard W., Antweiler E. & Tintelnot K. 2015. Molecular identification of fungal pathogens in nodular skin lesions of cats. Med Mycol, 53, 132–144.

Campbell J.J., Coyner K.S., Rankin S.C., Lewis T.P., Schick A.E. & Shumaker A.K. 2010. Evaluation of fungal flora in normal and diseased canine ears. Vet Dermat, 21, 619–625.

Chen W.T., Tu M.E. & Sun P.L. 2016. Superficial phaeohyphomycosis caused by Aureobasidium melanogenum mimicking Tinea Nigra in an immunocompetent patient and review of published reports. Mycopathologia, 181, 555–560.

De Oliveira L.R., Moraes-Souza H., Maltos A.L., Santos K.C., Molina R.J. & Barata C.H. 2013. Aureobasidium pullulans infection in a patient with chronic lymphocytic leukemia. Rev Soc Bras Med Trop, 46, 660–662.

Hofman V., Butori C., Long E., Fichoux Y.L. & Hofman P. 2008. Aureobasidium pullulans contamination in bronchial aspirates mimicking cryptococcosis: a rare diagnostic pitfall. Pathology, 40, 729–732.

Irinyi L., Serena C., Garcia-Hermoso D., Arabatzis M., Desnos-Ollivier M., Vu D., Cardinali G., Arthur I., Normand A.C., Giraldo A., da Cunha K.C., Sandoval-Denis M., Hendrickx M., Nishikaku A.S., de Azevedo Melo A.S., Merseguel K.B., Khan A., Parente Rocha J.A., Sampaio P., da Silva Briones M.R., Ferreira R.C., de Medeiros Muniz M., Castañón-Olivares L.R., Estrada-Barcenas D., Cassagne C., Mary C., Duan S.Y., Kong F., Sun A.Y., Zeng X., Zhao Z., Gantois N., Botterel F., Robbertse B., Schoch C., Gams W., Ellis D., Halliday C., Chen S., Sorrell T.C., Piarroux R., Colombo A.L., Pais C., de Hoog S., Zancopé-Oliveira R.M., Taylor M.L., Toriello C., de Almeida Soares C.M., Delhaes L., Stubbe D., Dromer F., Ranque S., Guarro J., Cano-Lira J.F., Robert V., Velegraki A. & Meyer W. 2015. International Society of Human and Animal Mycology (ISHAM)-ITS reference DNA barcoding database - the quality controlled standard tool for routine identification of human and animal pathogenic fungi. Med Mycol, 53, 313–337.

Joshi A., Singh R., Shah M.S., Umesh S. & Kattry N. 2010. Subcutaneous mycosis and fungemia by Aureobasidium pullulans: a rare pathogenic fungus in a post allogeneic BM transplant patient. Bone Marrow Transplantation, 45, 203–204.

Larone D.H. 2011. Aureobasidium pullulans. In Medically Important Fungi - A Guide to Identification (Larone D.H., ed.). 5th ed. ASM Press, Washington, 207–209.

Nenadović K., Vučinić M., Radenković-Damnjanović B., Janković L., Teodorović R., Voslarova E. & Becskei Z. 2017. Cortisol concentration, pain and sedation scale in free roaming dogs treated with carprofen after ovariohysterectomy. Vet World, 10, 888–894.

Perkins M.C., Martin P., Krockenberger M.B., Weeks K. & Malik R. 2004. Cerebral phaeohyphomycosis in a young female German shepherd. Aust Vet Pract, 34, 110–116.

Pikazis D., Xynos I.D., Xila V., Velegraki A. & Aronit K. 2009. Extended fungal skin infection due to Aureobasidium pullulans. Clin Exp Dermatol, 34, e892–e894.

Salkin I.F., Gordon M.A. & Stone W.B. 1976. Cutaneous infections of a porcupine (Erethizon dorsatum) by Aureobasidium pullulans. Sabouraudia, 14, 47–49.

Tamura K., Nei M. & Kumar S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA, 101, 11030–11035.