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An outbreak of duck hepatitis A virus infection in nomadic ducklings
VetIt_2590_16869_2

Keywords

Duck hepatitis A virus
Duckling
One‑step RT‑PCR

How to Cite

Annamalai, B., Jaisree, S., & Thandavan Vembuvizhivendan, T. (2022). An outbreak of duck hepatitis A virus infection in nomadic ducklings . Veterinaria Italiana, 58(4). https://doi.org/10.12834/VetIt.2590.16869.2

Abstract

During winter of the year 2020, a flock of 9 day‑old 5000 non‑descript ducklings was affected with huge daily mortality, dullness, depression and opisthotonus. Clinically, there was severe depression, spasmodic paddling and opisthotonus. On post‑mortem, liver was enlarged and pale with patchy ecchymoses. Presence of perihepatitis and pericardititis during post‑mortem examination of one duckling might be attributed to secondary bacterial infection. Upon completion of disease episode, there was 80 percent mortality in eight days and only less than 20 percent weak ducklings survived. Liver homogenate which was subjected for molecular confirmation through one‑step reverse transcriptase polymerase chain reaction (RT‑PCR) using primers for RNA dependent RNA polymerase (3D) gene yielded positivity for duck hepatitis A virus (DHAV‑1). Histological observation of liver revealed hepatocyte degeneration and necrosis. It is clear that DHAV‑1 which is epornitic in nature causes a major devastating disease endangering duck farming.

https://doi.org/10.12834/VetIt.2590.16869.2
VetIt_2590_16869_2

References

Asplin F.D. 1961. Notes on epidemiology and vaccination for virus hepatitis of ducks. Off Int Epizoot Bull, 56, 793-800.

Bancroft J.D. & Gamble M. 2008. Theory and practice of histological techniques. 6th Edn. Churchill Livingstone, Elsiever, London.

Fu Y., Pan M., Wang X., Xu Y., Yang H. & Zhang. D. 2008. Molecular detection and typing of duck hepatitis A virus directly from clinical specimens. Vet Microbiol, 131, 247–257.

Gazdzinski P. 1979. Attenuation of duck hepatitis virus and evaluation of its usefulness for duckling immunization. I. Studies on attenuation of the virus. Bull Vet Inst Pulawy, 23, 80–89.

Kamomae M., Kameyama M., Ishi J., Nabe M., Ogura Y., Iseki H., Yamamoto Y. & Mase M. 2017. An outbreak of duck hepatitis A virus type 1 infection in Japan. J Vet Med Sci, 79, 917-920.

Kim M., Kwan Y., Joh S., Kwon J., Kim J. & Kim S. 2007. Development of one-step RT-PCR to detect duck hepatitis virus type 1. Avian Dis, 51, 540-545.

Kim M., Kwon Y., Joh S., Kwon J. & Lindberg A. M. 2008. Differential diagnosis between type-specific duck hepatitis virus type 1 (DHV-1) and recent Korean DHV-1-like isolates using a multiplex polymerase chain reaction. Avian Pathol, 37, 171-177.

Kozydrun W., Czekaj H. & Lorek M. 2014. Outbreak of duck viral hepatitis in duckling flocks in Poland. Bull Vet Inst Pulawy, 58, 513 - 515

Hwang J. 1969. Duck hepatitis virus-neutralization in chicken embryo. Am J Vet Res, 30, 861-864.

Hisham I., Ellakany H.F., Selim A.A., Abdella M.A.M., Zain El-abideen M.A., Kilany W.H., Ali A. & Elbestawy A.R. 2020. Comparative pathogenicity of duck hepatitis virus type-1 isolates in experimentally infected Pekin and Muscovy ducks. Frontiers Vet Sci, 7, 234.

Levine P. P., & Fabricant J. 1950. A hitherto-undescribed virus disease of ducks in North America. Cornell Vet, 40, 71-86.

Murty D. K. & Hanson L.E. 1961. A modified microgel diffusion method and its application in the study of the virus of duck hepatitis. Am J Vet Res, 22, 274-278.

Niu Y., Ma H., Ding Y., Li Z., Sun Y., Li M. & Shi Y. 2019. The pathogenicity of duck hepatitis A virus types 1 and 3 on ducklings. Poult Sci, 98, 6333-6339.

Rao S.B.V. & Gupta B.R. 1967. Studies on a filterable agent causing hepatitis in ducklings, and biliary cirrhosis and blood dyscrasia in adults. Indian J Poult Sci, 2, 18–30

Rohaim M.A., Naggar R.F.E., AbdelSabour M.A., Ahmed B.A., Hamoud M.M., Ahmed K.A., Zahran O.K. & Munir M. 2021. Insights into the genetic evolution of duck hepatitis A virus in Egypt. Animals, 11(9), 2741

Sridhar G., Valliammai T., Varalakshmi C.S., Udayasankar K., Panchanadam M., Ramakrishna J., Gopal K.V., Jayaraman K. & Thyagarajan S.P. 1993. Duck hepatitis B virus (DHBV) infection in Indian domestic ducks: a pilot study. Trop Anim Health Prod, 25, 229-233.

Wold Organisation for Animal Health (OIE) Terrestrial Manual. 2018. Duck virus hepatitis. Chapter 3.3.8. Paris, OIE.

Yugo D.M., Hauck R., Shivaprasad H.L. & Meng X.J. 2016. Hepatitis virus infections in poultry. Avian Dis, 60, 576-588.

Zaho X., Phillips M.R., Li G. & Zhong A. 1991. Studies on the detection of antibody to duck hepatitis virus by enzyme-linked immuno-sorbent assay. Avian Dis, 35, 778-782.

Zanaty A., Hagag N., Samy M., Abdel-Halim A., Soliman M.A., Arafa A. & Nasif S. 2017. Molecular and pathological studies of duck hepatitis virus in Egypt. J Vet Med Res. 24, 374-384.

Zhang R., Yang Y., Lan J., Xie Z., Zhang X. & Jiang S. 2021. Evidence of possible vertical transmission of duck hepatitis A virus type 1 in ducks. Transboundary Emerg Dis, 68, 67-75.

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