Serological evidence of hepatitis E virus infection in pigs from Northern Bulgaria


Hepatitis E virus
Industrial farms
Northern Bulgaria

How to Cite

Tsachev, I. ., Baymakova, M., Dimitrov, K. K. ., Gospodinova, K. ., Marutsov, P. ., Pepovich, R. ., Kundurzhiev, T. ., Ciccozzi, M. ., & Dalton, H. R. . (2021). Serological evidence of hepatitis E virus infection in pigs from Northern Bulgaria. Veterinaria Italiana, 57(2), 155–159. https://doi.org/10.12834/VetIt.2341.14461.1


The purpose of the present study was to investigate pigs in Northern Bulgaria for serological evidence of hepatitis E virus (HEV). Sera from 225 individuals from three industrial farms were tested for anti‑HEV IgG antibodies. The overall HEV seroprevalence was 36% (81/225); weaners 6.8% (5/74); fattening pigs 38.7% (29/75) and in sows 61.8% (47/76). Compared to weaners, HEV positivity was higher in fattening pigs and sows: OR = 8.70 (95% CI: 3.14‑24.12) and OR = 22.37 (95% CI: 8.07‑61.96), respectively. These data confirm that HEV is endemic in pigs throughout Bulgaria, and can be a Public Health problem due to the transmission of HЕV to humans through the consumption of pork meat and pork products.



Arankalle V.A., Chobe L.P., Joshi M.V., Chadha M.S., Kundu B. & Walimbe A.M. 2002. Human and swine hepatitis E viruses from Western India belong to different genotypes. J Hepatol, 36, 417–425.

Baymakova M., Popov G.T., Pepovich R. & Tsachev I. 2019. Hepatitis E virus infection in Bulgaria: a brief analysis of the situation in the country. Open Access Maced J Med Sci, 7, 458–460.

Baymakova M., Sakem B., Plochev K., Popov G.T., Mihaylova-Garnizova R., Kovaleva V. & Kundurdjiev T. 2016. Epidemiological characteristics and clinical manifestations of hepatitis E virus infection in Bulgaria: a report on 20 patients. Srp Arh Celok Lek, 144, 63–68.

Breum S.O., Hjulsager C.K., de Deus N., Segales J. & Larsen L.E. 2010. Hepatitis E virus is highly prevalent in the Danish pig population. Vet Microbiol, 146, 144–149.

Bruni R., Villano U., Equestre M., Chionne P., Madonna E., Trandeva-Bankova D., Peleva-Pishmisheva M., Tenev T., Cella E., Ciccozzi M., Pisani G., Golkocheva-Markova E. & Ciccaglione A.R. 2018. Hepatitis E virus genotypes and subgenotypes causing acute hepatitis, Bulgaria, 2013-2015. PLoS One, 13, e0198045.

Cella E., Golkocheva-Markova E., Sagnelli C., Scolamacchia V., Bruni R., Villano U., Ciccaglione A.R., Equestre M., Sagnelli E., Angeletti S. & Ciccozzi M. 2019. Human hepatitis E virus circulation in Bulgaria: Deep Bayesian phylogenetic analysis for viral spread control in the country. J Med Virol, 91, 132–138.

European Association for the Study of the Liver (EASL). 2018. EASL Clinical Practice Guidelines on hepatitis E virus infection. J Hepatol, 68, 1256–1271.

European Centre for Disease Prevention and Control (ECDC). 2017. Hepatitis E in the EU/EEA, 2005–2015. Stockholm (ECDC).

Garcia-Hernandez M.E., Cruz-Rivera M., Sanchez-Betancourt J.I., Rico-Chavez O., Vergara-Castaneda A., Trujillo M.E. & Sarmiento-Silva R.E. 2017. Seroprevalence of anti-hepatitis E virus antibodies in domestic pigs in Mexico. BMC Vet Res, 13, 289.

Hsieh S.Y., Meng X.J., Wu Y.H., Liu S.T., Tam A.W., Lin D.Y. & Liaw Y.F. 1999. Identity of a novel swine hepatitis E virus in Taiwan forming a monophyletic group with Taiwan isolates of human hepatitis E virus. J Clin Microbiol, 37, 3828–3834.

International Committee on Taxonomy of Viruses (ICTV). 2018. Hepeviridae (Virus Taxonomy: 2018b Release). https://talk.ictvonline.org (accessed on July 20, 2020).

Jemersic L., Keros T., Maltar L., Barbic L., Vilibic-Cavlek T., Jelicic P., Rode O.D. & Prpic J. 2017. Differences in hepatitis E virus (HEV) presence in naturally infected seropositive domestic pigs and wild boars – an indication of wild boars having an important role in HEV epidemiology. Vet Arhiv, 87, 651–663.

Lupulovic D., Lazic S., Prodanov-Radulovic J., Jimenez de Oya N., Escribano-Romero E., Saiz J.C. & Petrovic T. 2010. First serological study of hepatitis E virus infection in backyard pigs from Serbia. Food Environ Virol, 2, 110–113.

Martinelli N., Luppi A., Cordioli P., Lombardi G. & Lavazza A. 2011. Prevalence of hepatitis E virus antibodies in pigs in Northern Italy. Infect Ecol Epidemiol, 1, 10.3402/iee.v1i0.7331.

Meng X.J., Dea S., Engle R.E., Friendship R., Lyoo Y.S., Sirinarumitr T., Urairong K., Wang D., Wong D., Yoo D., Zhang Y., Purcell R.H. & Emerson S.U. 1999. Prevalence of antibodies to the hepatitis E virus in pigs from countries where hepatitis E is common or is rare in the human population. J Med Virol, 59, 297–302.

Pavio N., Meng X.J. & Renou C. 2010. Zoonotic hepatitis E: animal reservoirs and emerging risks. Vet Res, 41 (6), 46.

Pepovich R., Baymakova M., Pishmisheva M., Marutsov P., Pekova L. & Tsachev I. 2019. Current knowledge on hepatitis E virus infection. Vojnosanit Pregl, 76, 733–739.

Pishmisheva M., Baymakova M., Golkocheva-Markova E., Kundurzhiev T., Pepovich R., Popov G.T. & Tsachev I. 2018. First serological study of hepatitis E virus infection in pigs in Bulgaria. C R Acad Bulg Sci, 71, 1001–1008.

Purdy M.A., Harrison T.J., Jameel S., Meng X.J., Okamoto H., Van der Poel W.H.M., Smith D.B. & ICTV Report Consortium. 2017. ICTV virus taxonomy profile: Hepeviridae. J Gen Virol, 98, 2645–2646.

Rose N., Lunazzi A., Dorenlor V., Merbah T., Eono F., Eloit M., Madec F. & Pavio N. 2011. High prevalence of hepatitis E virus in French domestic pigs. Comp Immunol Microbiol Infect Dis, 34, 419–427.

Savuta G., Anita A., Anita D., Ludu L. & Pavio N. 2007. Preliminary epidemiological investigations regarding hepatitis E virus infection in swine from the North-East of Romania. Bulletin USAMV-CN, 64, 356–358.

Tam A.W., Smith M.M., Guerra M.E., Huang C.C., Bradley D.W., Fry K.E. & Reyes G.R. 1991. Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome. Virology, 185, 120–131.

Teoharov P., Tiholova M., Draganov P., Lilyanova V., Ivanova R., Varleva T. & Dimitrova T. 1995. First cases of hepatitis E virus infection in Bulgaria. Infectology (Sofia), 32, 17–18.

Tsachev I., Baymakova M., Ciccozzi M., Pepovich R., Kundurzhiev T., Marutsov P., Dimitrov K.K., Gospodinova K., Pishmisheva M. & Pekova L. 2019. Seroprevalence of hepatitis E virus infection in pigs from Southern Bulgaria. Vector Borne Zoonotic Dis, 19, 767–772.

Withers M.R., Correa M.T., Morrow M., Stebbins M.E., Seriwatana J., Webster W.D., Boak M.B. & Vaughn D.W. 2002. Antibody levels to hepatitis E virus in North Carolina swine workers, non-swine workers, swine, and murids. Am J Trop Med Hyg, 66, 384–388.