Abstract
This study aimed to investigate environmental mastitis causing bacteria counts in the teat end, somatic cell counts (SCC) of milk samples, cleanliness scores and behavior of cows kept on concrete and rubber mat floorings. For this purpose, 19 Holstein-Friesian dairy cows were allocated into concrete and rubber mat groups. Swab samples were taken from the teat ends to determine the bacterial counts causing environmental mastitis. Milk samples were collected from a composite of all four quarters to determine the SCC. Instantaneous sampling method was utilized to observe the behavioral activities of cows. Cows were visually evaluated to determine the udder cleanliness score. Independent samples t-test was utilized in the statistical analysis of the obtained data. Coliform (P<0.05), Escherichia coli, and Klebsiella spp. (P<0.01) counts of the swab samples taken from the cows housed on concrete flooring were significantly higher than rubber mat group. However, no statistically significant differences were found between groups in terms of total bacteria, Streptococcus spp., and Enterobacteriaceae counts. The SCC on samples taken from cows kept on concrete surface were significantly higher (P<0,05) than that of animals housed on rubber mat. Furthermore, cows in the rubber mat group were determined to be significantly cleaner (P<0.05) than those in concrete group. It was also determined that the cows housed on rubber mat spent significantly longer time for lying behavior (P<0.05), which is a significant indicator of animal comfort. The time spent for standing without eating was considerably higher (P<0.01) in concrete group. In addition, the times spent for eating was significantly lower (P<0.01) in the concrete group. It was concluded that, using rubber mat instead of concrete for flooring in tie-stall barns decreases the contamination of environmental mastitis pathogens, increases milk quality and cow cleanliness score as well as animal comfort and welfare.
References
Anderson N.G. 2001. Time lapse video opens our eyes to cow comfort and behavior. Proc American Assoc Bovine Practitioners Conference, 34, 35-42.
Büyükkök, S., Büyüktaş, K., Tezcan, A. & Karaca, C. 2019. The effect of different bedding materials used on free-stalls on dairy cows’ preferences. Large Anim Rev, 25, 119-126.
Botrel, M.A., Haenni, M., Morignat, E., Sulpice, P., Madec, J.Y. & Cala-vas, D. 2010. Distribution and antimicrobial resistance of clinicaland subclinical mastitis pathogens in dairy cows in Rhone-Alpes,France. Foodborne Patho Dis, 7, 479–487.
Calamari, L., Calegari, F. & Stefanini, L. 2009. Effect of different free stall surfaces on behavioural, productive and metabolic parameters in dairy cows. Appl Anim Behav Sci, 120, 9-17.
Chen, C.S., Liau, W.Y., & Tsai, G.J. 1998. Antibacterial effects of N-sulfonated and N-sulfobenzoyl chitosan and application to oyster preservation. J Food Prot, 61, 1124-1128.
Desmasures, N.; Bazin, F. and Gueguen, M. (1997): Microbiological composition of raw milk from selected farms in the Camembert region of Normandy. J Appl Microbiol, 83, 53-58.
Erdem, H., & Okuyucu, I. C. (2019). Influence of hygiene status of cows on somatic cell count and milk components during summer season. Large Anim Rev, 25, 7-10.
Gautam. V.N., Shraddha Shrivastava, G.P. Lakhani, Sahehar R. & Tripathi K.N. 2020. A Review: IMPACT of Floor Type and Bedding Materials on Performance of Dairy Animals. Int J Curr Microbiol App Sci. 9,1524-1534.
Graunke, K.L., Telezhenko, E., Hessle, A. K., Bergsten, C. & Loberg J.M. 2011. Does rubber flooring improve welfare and production in growing bulls in fully slatted floor pens? Animal Welfare, 20, 173-183.
Halasa, T. & Kirkeby, C. 2020. Differential somatic cell count: Value for udder health management. Front Vet Sci, 7, 1153.
Haley, D.B., de Passille´, A.M & Rushen. J. 2001. Assessing cow comfort: Effects of two floor types and two tie stall designs on the behaviour of lactating dairy cows. Appl Anim Behav Sci, 71, 105–117.
Hogan, J.S., Smith, K.L., Hoblet, K.H., Todhunter, D.A., Schoenberger, P.S., Hueston, W.D., Pritchard, D.E., Bowman G.L., Heider, L.E., Brockett B.L. & Conrad, H.R. 1989. Bacterial counts in bedding materials used on nine commercial dairies. J Dairy Sci, 72, 250-258.
Hogan, J.S., Bogacz V.L., Thompson L.M., Romig S., Schoenberger P.S., Weiss W.P. &. Smith K.L. 1999. Bacterial counts associated with sawdust and recycled manure bedding treated with commercial conditioners. J Dairy Sci, 82, 1690–1695.
Karakok, S. G., Uslucan, B., Tapki, I., & Gokce, G. (2009). The effect of straw bedding usage in loose housing systems on behavior and milk production of Holstein dairy cows. J Anim Vet Adv, 8, 1824-1828.
Kartal, T.Z. & Yanar, M. 2011. Effect of floor types on the growth performance and some behavioural traits of Brown Swiss calves. Vet ir Zootech, 55, 20-24.
Klaas, I.C. & Zadoks, R.N. 2017. An update on environmental mastitis: Challenging perceptions. Transbound Emerg Dis, 65, 166-185.
Kristula, M.A., Dou, Z., Toth, J.D., Smith, B.I., Harvey, N. & Sabo, M. 2008. Evaluation of free-stall mattress bedding treatments to reduce mastitis bacterial growth. J Dairy Sci, 91, 1885-1892.
Kumar, A., Kamboj, M.L., Chandra, S. & Bharti, P. 2017. Effect of modified housing system on physiological parameters of Murrah buffaloes during autumn and winter season. Indian J Anim Res, 52, 829-833.
Levison, L.J., Miller-Cushon, E.K., Tucker, A.L., Bergeron, R., Leslie, K.E., Barkema, H.W. & DeVries, T.J. 2016. Incidence rate of pathogen-specific clinical mastitis on conventional and organic Canadian dairy farms. J Dairy Sci, 99, 1341-1350.
Massé, J., Dufour, S. & Archambault, M. 2020. Characterization of Klebsiella isolates obtained from clinical mastitis cases in dairy cattle. J Dairy Sci, 103, 3392-3400.
Munoz, M.A., Bennett, G.J., Ahlström, C., Griffiths, H.M., Schukken, Y.H. & Zadoks, R.N. 2008. Cleanliness scores as indicator of Klebsiella exposure in dairy cows. J Dairy Sci, 91, 3908-3916.
Natzke, R.P., and B.J. LeClair. 1976. Coliform contaminated bedding and new infections. J Dairy Sci, 59, 2152–2154.
Neja, W., Bogucki, M., Jankowska, M. & Sawa, A. 2016. Effect of cow cleanliness in different housing systems on somatic cell count in milk. Acta Veterinaria Brno, 85, 55-61.
Norring, M., Manninen, E., De Passillé, A.M., Rushen, J., Munksgaard, L. & Saloniemi, H. 2008. Effects of sand and straw bedding on the lying behavior, cleanliness, and hoof and hock injuries of dairy cows. J Dairy Sci, 91, 570-576.
Pacini, F., Cariolato, D., Andrighetto, C. & Lombardi, A. 2006. Occurrence of Streptococcus macedonicus in Italian cheeses. FEMS microbiology letters, 261, 69-73.
Paduch, J.H., Mohr, E. & Krömker, V. 2013. The association between bedding material and the bacterial counts of Staphylococcus aureus, Streptococcus uberis and coliform bacteria on teat skin and in teat canals in lactating dairy cattle. J Dairy Sci, 80, 159-164.
Paduch, J.H., Mohr, E. & Krömker, V. 2013. The association between bedding material and the bacterial counts of Staphylococcus aureus, Streptococcus uberis and coliform bacteria on teat skin and in teat canals in lactating dairy cattle. J Dairy Res, 80, 159-164.
Proietto, R.L., Hinckley, L.S., Fox, L.K. & Andrew, S.M. 2013. Evaluation of a clay-based acidic bedding conditioner for dairy cattle bedding. J Dairy Sci, 96, 1044-1053.
Reneau, J.K., Seykora, A.J., Heins, B.J., Endres, M.I., Farnsworth, R.J. & F. Bey, R. 2005. Association between hygiene scores and somatic cell scores in dairy cattle. J Am Vet Med Assoc, 227, 1297-1301.
Sant’Anna, A.C. & da Costa, M.P. 2011. The relationship between dairy cow hygiene and somatic cell count in milk. J Dairy Sci, 94, 3835-3844.
Schukken, Y., Chuff, M., Moroni, P., Gurjar, A., Santisteban, C., Welcome, F. & Zadoks, R. 2012. The “other” gram-negative bacteria in mastitis: Klebsiella, Serratia, and more. Vet Clin N Am - Food Anim Pract, 28, 239-256.
Singh, A., Kumari, T.,Rajput, M., Baishya, A., Bhatt, N. & Roy, S. 2020. A Review: Effect of Bedding Material on Production, Reproduction and Health and Behavior of Dairy Animals. Int J Livest Res, 10, 11-20.
SPSS, I. 2011. IBM SPSS statistics for Windows, version 20.0. New York: IBM Corp 440.
Suojala, L., Kaartinen, L. & Pyörälä, S. 2013. Treatment for bovine E scherichia coli mastitis–an evidence‐based approach. J Vet Pharmacol Ther, 36, 521-531.
Thomsen, P.T., Munksgaard, L. & Sørensen, J.T. 2012. Locomotion scores and lying behaviour are indicators of hoof lesions in dairy cows. The Veterinary Journal, 193, 644–647.
Ward, W.R., Hughes J.W., Faull W.B., Cripps P.J., Sutherland J.P. & Sutherst J. E. 2002. Observational study of temperature, moisture, pH and bacteria in straw bedding, and faecal consistency, cleanliness and mastitis in cows in four dairy herds. Vet Rec, 151, 199–206.
Zdanowicz, M., Shelford, J.A., Tucker, C.B., Weary, D.M. & Von Keyserlingk, M.A.G. 2004. Bacterial populations on teat ends of dairy cows housed in free stalls and bedded with either sand or sawdust. J Dairy Sci, 87, 1694-1701.