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Molecular Detection of Peste des Petits Ruminants Virus (PPRV) in Goats and in Sheep in Ibadan, Oyo State, Nigeria
VetIt_2558_15754_1

Keywords

Goats
Ibadan
Peste des petits ruminants virus
Sheep
Vaccine

How to Cite

Adeoye, F., Kolapo, A., Ogunmolawa, O., Aluko, A., Meseko, C., & Oluwayelu, D. (2022). Molecular Detection of Peste des Petits Ruminants Virus (PPRV) in Goats and in Sheep in Ibadan, Oyo State, Nigeria. Veterinaria Italiana, 58(4). https://doi.org/10.12834/VetIt.2558.15754.1

Abstract

Peste des petits ruminants (PPR) is a vaccine-preventable transboundary animal disease of goats and sheep majorly, and is regarded as a major constraint to small ruminant production especially in developing countries like Nigeria. Despite different strategies that have been employed to control PPR in Nigeria, cases of the disease are still reported in PPR-vaccinated and unvaccinated small ruminant farms. In this study, molecular detection of field PPR virus (PPRV) strains was carried out to determine the presence of PPRV. A total of 135 samples (45 oculo-nasal swabs and 90 tissue samples) were purposively collected between August and October 2020 from goats and sheep at the Akinyele live small ruminant market and at Akinyele and Amosun abattoirs in Ibadan, Oyo State, Nigeria. Using reverse transcriptasepolymerase chain reaction with primers targeting the partial N-gene of PPRV, 10 out of the 135 (7.4%) field samples yielded positive results. The results of this study reveal that PPRV currently circulates in Ibadan. These findings underscore the need for continuous PPR surveillance, more extensive characterization of circulating PPRV strains and the importance of consistent use of quality vaccines in the country to achieve more effective preventive and control strategies against the disease.

https://doi.org/10.12834/VetIt.2558.15754.1
VetIt_2558_15754_1

References

Abraham G., Libeau G., Roger F., Laekemariam Y., Abayneh D. & Awoke K.M. 2005. Antibody seroprevalences against peste des petits ruminants (PPR) virus in camels, cattle, goats and sheep in Ethiopia. Prev Vet Med, 70, 51–57.

Abubakar M., Ali Q. & Khan H.A. 2008. Prevalence and mortality rate of peste des petits ruminant (PPR) possible association with abortion in goat. Trop Animal Health Prod, 40, 317–321.

Adamu S., Ekundayo O.S., Useh N.M., Neils J.S., Bissallah M. & Esievo K.A.N. 2005. Serum levels of some Electrolytes in diarrhoeic Savanna nd Brown goats in Zaria. In 42nd Congress of Nigerian Veterinary Medical Association, 14 -18 November, Maiduguri, Nigeria.

Alemu B. , Gari G., Libeau G., Kwiatek O., Kidane M., Belayneh R., Siraw B., Wieland B., Asfaw W. & Abdi R. 2019. Molecular detection and phylogenetic analysis of Peste des petits ruminants virus circulating in small ruminants in eastern Amhara region, Ethiopia. BMC Vet Res, 15, 84.

Baazizi R., Mahapatra M., Clarke B.D., Ait- Oudhia K., Khelef D. & Parida S. 2017. Peste despetits ruminants (PPR): a neglected tropical disease in Maghreb region of North Africa and its threat to Europe. PLoS ONE, 12, e0175461.

Balogun F.A, Fasanmi O.G., Oladipo T.A, Popoola M.A, Olona J.F. & Adeoye Y.D. 2017. Field evaluation and confirmation of acute peste des petits ruminant outbreak in a flock of West African dwarf goats in Ibadan, Nigeria. Int J Vet Sci Med, 5 (2),175-180,

Banyard A., Parida S., Batten C., Oura C., Kwiatek O. & Libeau G. 2010. Global distribution of peste des petits ruminants virus and prospects for improved diagnosis and control. J Gen Virol, 91, 2885–2897.

Banyard A., Wang Z. & Parida S. 2014. Peste des Petits Ruminants Virus, Eastern Asia. Emerg Inf Dis, 20, 2176–2177.

Bidjeh K., Bornarel P., Imadine M. & Lancelot R. 1995. First time isolation of the PPR virus in Chad and experimental induction of the disease Premier isolement au Tchad du virus de la PPR et reproduction experimentale de la maladie. Rev Elev Med Vet Pays Trop, 48, 295–300.

Cattaneo, R., Rebmann, G., Baczko, K., Ter Meulen, V. & Billeter, M.A. 1987. Altered ratios of measles virus transcripts in diseased human brains. Virology, 160, 523–526.

Chauhan H., Chandel B., Kher H., Dadawala A. & Agrawal S. 2009. Peste des petits ruminants infection in animals. Vet World, 2, 150–155.

Couacy-Hymann E., Bodjo S., Danho T., Koffi M., Libeau G. & Diallo A. 2007. Early detection of viral excretion from experimentally infected goats with peste-des-petits ruminants virus. Prev Vet Med, 78, 85–88.

Couacy-Hymann E., Roger F., Hurard C., Guillou J.P., Libeau G. & Diallo A. 2002. Rapid and sensitive 100, detection of peste des petits ruminants virus by a polymerase chain reaction assay. J Virol Methods, 100, 17–25.

De Nardi M., Lamin Saleh S.M., Batten C., Oura C., Di Nardo A. & Rossi D. 2012. First evidence of Peste des Petits ruminants (PPR) virus circulation in Algeria (Sahrawi territories): outbreak investigation and virus lineage identification. Transbound Emerg Dis, 59, 214–222.

Diop M., Sarr J. & Libeau G. 2005. Evaluation of novel diagnostic tools for peste des petits ruminants virus in naturally infected goat herds. Epidemiol Infect, 133, 711–717.

Ezeibe M., Okoroafor O., Ngene A., Eze J. & Eze I. 2008. Persistent detection of peste de petits ruminants antigen in the faeces of recovered goats. Trop Animal Health, 40, 517–519.

Food and Agricultural Organisation of the United Nations (FAO). 2006. FAOSTAT database. Food and Agricultural Organisation, Rome, Italy.

Food and Agriculture Organizations of the United Nations (FAO). 1999: Recognizing Peste des Petits Ruminants. A Field manual (P.L Roeder & T.U. Obi, eds). https://www.fao.org/3/x1703e/x1703e00.htm

George A.A. 2002. Comparative evaluation of different gene targets for PCR diagnosis of PPR. M. V. Sc. Thesis, Deemed University IVRI, Izatuagar, India.

Hamdy F.M. & Dardiri A.H. 1976. Response of white-tailed deer to infection with peste des petits ruminants virus. J Wildl Dis, 12, 516–522.

Kazeem H.M., Shamaki D., Ibru J.O., Fasina F.O., Aba-Adulugba E.P., Bitrus Y., Owolodun O.A., Shuaibu S., Tyem A.D., Ogedengbe M.F., Antiabong J.F. & Lombin L.H. 2009. 2008 Field outbreak of peste des petits ruminants (PPR) in sheep and goats from different geo-ecological zones of Nigeria. In 46th Annual Conference of Nigerian Veterinary Medical Association, 19-23 October, Anambra, Nigeria.

Kerur N., Jhala M.K. & Joshi C.G. 2008. Genetic characterization of Indian peste des petits ruminants virus (PPRV) by sequencing and phylogenetic analysis of fusion protein and nucleoprotein gene segments. Res Vet Sci, 85(1),176-183.

Kgotiele T., Macha E.S., Kasanga C.J., Kusiluka L.J.M., Karimuribo E.D., Van Doorsselaere J., Wensman J.J., Munir M. & Misinzo G. 2014. Partial genetic characterization of Peste des petits ruminants virus from goats in northern and eastern Tanzania. Transbound Emerg Dis, 61(1), 56–62.

Kul O., Kabakci N., Atmaca H. & Ozkul A. 2007. Natural peste des petits ruminants virus infection: novel pathologic findings resembling other morbillivirus infections. Vet Pathol, 44(4), 479-486.

Kwiatek O., Ali Y.H., Saeed I.K., Khalafalla A.I., Mohamed O.I., Obeida A.A., Abdelrahman M.B.,Osman H.M., Taha K.M., Abbas Z., El Harrak M., Lhor Y., Diallo A., Lancelot R., Albina E. & Libeau G. 2011. Asian lineage of Peste des Petits ruminants virus, Africa. Emerg Infect Dis, 17(7),1223–1231.

Luka P., Erume J., Mwiine F.N., Ayebazibwe C. & Shamaki D. 2011. Molecular characterization and phylogenetic study of peste des petits ruminants viruses from North central States of Nigeria. BMC Vet Res, 4, 7.

Luka P.D., Ayebazibwe C., Shamaki D., Mwiine F.N. & Erume J. 2012. Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR. J Vet Sci, 13, 323–325.

Mahajan S., Agrawal R., Kumar M., Mohan A. & Pande N. 2013. Incidence of Peste des petits ruminants in nomadic sheep and goat of Jammu region. Vet World, 6(7), 384–387.

Mantip S., Quan M., Shamaki D. & Van Vuuren M. 2016. Comparison of nucleotide sequences of recent and previous lineages of peste-des-petits-ruminants viruses of sheep and goats in Nigeria. Onderstepoort J Vet Res, 83(1), a1163.

Obi T.U. & Ojeh C.K. 1989. Dot enzyme immunoassay for visual detection of peste des- petits-ruminants virus antigen from infected caprine tissues. J Clin Microbiol, 27(9), 2096-2099.

Radostits O.M., Blood D.C. & Gay C.C. 2000. Veterinary Medicine. A textbook of the diseases of the cattle, sheep, pigs, goats and horses. 9 ed., W.B. Saunders, London, UK.

Saliu O., Audu S., Sanda M., Aribido S. & Olaolu M. 2008. Adoption of vaccination and ethnoveterinary treatment for peste des petits ruminnats (PPR) among sheep and goat farmers in Ijumu local Government area of Kogi State. Nig Agric J, 3(5), 404-408.

Shamaki D., Olaleye O.D., Obi T. U., Diallo A., Majiyagbe K.A., Lombin L.H. & Barrett T. 2004. Peste Des petits Ruminants (PPR) in Nigeria: Serological and Molecular Epidemiology. Vom J VetSci, 1: 18 – 27.

Thrusfield M. & Christley R. 2005. Veterinary epidemiology. Wiley Online Library.

Ularamu H.G., Owolodun O.A., Woma T.Y., Audu B.J, Aaron G.B, Chollom S.C. & Shamaki D. 2012. Molecular diagnosis of recent suspected outbreaks of peste des petits ruminants (PPR) in Yola, Adamawa State, Nigeria. African J Biotechnol, 11(5), 1158-1162.

Wang J., Bao X., Wu Y., Liu L., Li C. & Liu L. 2009. Peste des petits ruminants virus in Tibet, China. Emerg Infect Dis 15, 299–301.

Wells J. C. 2008. In Longman Pronunciation Dictionary (3rd ed.). Longman. ISBN978-1-4058-8118-0

World Organisation for Animal Health (OIE). 2009. OIE manual of Diagnostic Tests and vaccines for Terrestrial Animals. Paris, OIE.

Wosu L.O. 1994. Current status of peste des petits ruminants (PPR) disease in small ruminants. A review article. Stud Res Vet Med, 2, 83–90.

Yesilbag K., Yilmaz Z., Golcu E. & Ozkul A. 2005. Peste des petits ruminants outbreak in Western Turkey. Vet Rec, 157, 260–261.

Copyright (c) 2023 Fiyinfoluwa Adeoye, Dr Fiyinfoluwa Adeoye, Dr Adedamilola Kolapo, Oluyemi Ogunmolawa, Adegboyega Aluko, Dr Clement Meseko, Professor Oluwayelu