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O4-2 Antibody response elicited by Brucella abortus strain RB51 vaccine in young water buffaloes (Bubalus bubalis) using a triple dose and the vaccination schedule authorised in Caserta province, Italy
2022, 4 - Immunology
2022

O4-2 Antibody response elicited by Brucella abortus strain RB51 vaccine in young water buffaloes (Bubalus bubalis) using a triple dose and the vaccination schedule authorised in Caserta province, Italy

4 - Immunology
Published 2023-02-10
Flavio Sacchini
WOAH and National Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy
Fabrizio De Massiss
WOAH and National Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy
Nicola D'Alterio
WOAH and National Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy
Diana Rodomonti
WOAH and National Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy
Chiara Di Pancrazio
WOAH and National Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy
Tiziana Di Febo
WOAH and National Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy
Annarita Natali
WOAH and National Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy
Nicola Ferri
WOAH and National Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy
Emanuela Rossi
WOAH and National Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy
Giovanni Befacchia
WOAH and National Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy
Giacomo Vincifori
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy
Simona Iannetti
WOAH and National Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy
Manuela Tittarelli
WOAH and National Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy

Keywords

Brucellosis
Water buffalo
RB51
immune response

Categories

Abstract

Eradication of animal brucellosis is still a priority in Italy and the disease has a relevant impact on water buffaloes in the province of Caserta, Campania Region, Italy. In order to reduce the prevalence of infection, vaccination with the live attenuated vaccine B. abortus strain RB51 has been authorized by Central Veterinary Authority (Ministry of Health) in water buffalo herds in endemic territories, under controlled conditions. Namely, vaccination of young animal only (6-8 months old females, before sexual maturity), using a dose 3 times higher than the one used for cattle, followed by a second injection of same dose, 1 month after the first administration. In fact, when used on adult animals RB51 can be excreted in milk or induce abortion in pregnant animals. The aim of the study was to acquire information on development and persistence of RB51 specific antibodies induced by the vaccination schedule authorized from the time of vaccination (6-8 months old) to the stage of adult (26-28 months old). 22 buffaloes 7 - 8 months old were vaccinated with the commercial vaccine RB51 CZ Veterinaria, S.A. (Spain) using a triple dose. Four buffaloes were included as controls, inoculated with placebo and immediately mixed with vaccinated animals. A booster vaccination was administered after 30 days. Blood samples were collected regularly after vaccination. Sera were tested using a specific Complement Fixation test performed using RB51 antigen (RB51-CFT). RB51- CFT identified as positive 80% of the animals 9 days after first vaccination. Following booster vaccination, all vaccinated animals tested positive for approximately 2 months. Compared to our previous studies, results suggest that age of vaccination influences antibody response and RB51-CFT results. Two months after booster vaccination, antibody titers and numbers of vaccinated animals testing positive for RB51-CFT was variable and decreased in a non-progressive trend. Actually, we observed that several animal alternated positive and negative results to RB51-CFT up to 18 months after booster vaccination. This information represent a relevant element to develop diagnostic protocols to survey and detect the possible unauthorised application of RB51 vaccination on adult animals.

References

Iovane G., Martucciello A., Astarita S., Galiero G., Pasquali P., Adone R., Ciuchini F., Pagnini U., Guarino A., Fusco G. (2007) Vaccino Brucella abortus RB51. Il Progresso Veterinario, 1: 19-21.

Tittarelli M, Atzeni M, Calistri P, Di Giannatale E, Ferri N, Marchi E, Martucciello A, De Massis F. (2015). A diagnostic protocol to identify water buffaloes (Bubalus bubalis) vaccinated with Brucella abortus strain RB51 vaccine. Vet Ital. 51(2):99-105.